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KMID : 0364820120480020125
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Korean Journal of Microbiology
2012 Volume.48 No. 2 p.125 ~ p.133
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Microbial Diversity in Three-Stage Methane Production Process Using Food Waste
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Nam Ji-Hyun
Kim Si-Wouk
Lee Dong-Hun
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Abstract
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Anaerobic digestion is an alternative method to digest food wastes and to produce methane that can be used as a
renewable energy source. We investigated bacterial and archaeal community structures in a three-stage methane
production process using food wastes with concomitant wastewater treatment. The three-stage methane process is
composed of semianaerobic hydrolysis/acidogenic, anaerobic acidogenic, and strictly anaerobic methane production
steps in which food wastes are converted methane and carbon dioxide. The microbial diversity was determined by
the nucleotide sequences of 16S rRNA gene library and quantitative real-time PCR. The major eubacterial
population of the three-stage methane process was belonging to VFA-oxidizing bacteria. The archaeal community
consisted mainly of two species of hydrogenotrophic methanogen (Methanoculleus). Family Picrophilaceae (Order
Thermoplasmatales) was also observed as a minor population. The predominance of hydrogenotrophic methanogen
suggests that the main degradation pathway of this process is different from the classical methane production
systems that have the pathway based on acetogenesis. The domination of hydrogenotrophic methanogen
(Methanoculleus) may be caused by mesophilic digestion, neutral pH, high concentration of ammonia, short HRT,
and interaction with VFA-oxidizing bacteria (Tepidanaerobacter etc.).
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KEYWORD
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archaeal diversity, bacterial diversity, hydrogenotrophic methanogen, three-stage methane production process, VFA-oxidizing bacteria
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